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tRNA suppressor mutation to transcribe nonsense mutations (lemm.ee)

submitted 4 months ago* (last edited 4 months ago) by [email protected] to c/[email protected]

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Okay, so I know this might be a bit hyperspecific, but I don't know where else to ask it. I'm working through a microbiology lecture, and the professor says the the B strain of E. coli has a tRNA suppressor that allows it to transcribe phage genes that have any nonsense mutation. That seemed a bit vague, so I decided to look it up. But the only thing I can find that's even remotely similar is that that strain doesn't express T7 RNA polymerase, which doesn't seem terribly helpful. Is there anything like this in that particular strain? It seems like a load of bs to me that a bacterium should just be able to ignore any stop codon.

Edit: My prof might have been referring specifically to an amber mutation. So, just one stop codon. Seems my resources are just poorly worded.

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[–] [email protected] 4 points 4 months ago

It's useful for lab applications, that's not the part I'm worried about.